Cross-linking of dermal sheep collagen using a water-soluble carbodiimide

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Cross-linking of dermal sheep collagen using a water-soluble carbodiimide.

A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC). Cross-linking using EDC involves the activation of carboxylic acid groups to give O-acylisourea groups, which form cross-links after reaction with free amine groups. Treatment of dermal sheep collagen (DSC) with EDC (E-DSC)...

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In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide.

Bacterial collagenase was used to study the susceptibility of dermal sheep collagen (DSC) cross-linked with a mixture of the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide (E/N-DSC) towards enzymatic degradation. Contrary to non-cross-linked DSC (N-DSC), which had a rate of weight-loss of 18.1% per hour upon degradation, no weig...

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Histopathological Finding of Cornea after Collagen Cross-Linking Using Riboflavin and Ultraviolet A in Rabbit

Purpose: To evaluate the histopathological changes of the cornea in rabbit corneas after accelerated collagen cross-linking. Methods: Right eyes of 7 adult New Zealand albino rabbits weighing 1.5 to 2.0 kg were studied. Right eyes of all animals were in the treatment group and left eyes were in the control group. The epithelium of all right eyes was removed and was crossed-linked with riboflavi...

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collagen cross-linking

patients with keratoconus constitute a large proportion of patients suffering from anterior segment disorders. as of this time, however, its pathogenesis has not yet been elucidated. a reduction in cross-link between collagen molecules has been observed in keratoconic eyes as compared to normal ones. present approaches only address refractive errors resulting from the abnormal abnormal corneal ...

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Effect of Solvents on Dentin Collagen Cross-linking Potential of Carbodiimide.

PURPOSE This study compared the dentin collagen cross-linking potential of carbodiimide (EDC) prepared in three most commonly used solvents in dental adhesive systems: water, ethanol, and acetone. MATERIALS AND METHODS Thirty-eight extracted caries-free human permanent molars were used in this study. Demineralized dentin beams were prepared and cross linked by 0.3 M EDC in water, acetone, or ...

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ژورنال

عنوان ژورنال: Biomaterials

سال: 1996

ISSN: 0142-9612

DOI: 10.1016/0142-9612(96)81413-x